A number of security guards quickly intervened to separate the two boxers after the incident, which occurred at AT&T Stadium in Texas.
The 58-year-old Mike Tyson – who, wearing only a pair of Versace luxury brand underwear, had weighed in at just over 103.6 kilos shortly beforehand – was brief.
The talk is over, he said before leaving the scene with his team.
The challenger Jake Paul, the 27-year-old YouTuber turned boxer, weighed about half a kilo less.
I didn't even feel it, said Paul about the slap.
He's angry, he's a little angry elf. Cute slap, buddy.
Paul promised to defeat Tyson and ended with a theatrical roar into the microphone that Tyson "must die".
Jake Paul and Mike Tyson will meet in a highly publicized match on Saturday night, Swedish time. Both are expected to earn enormous sums from the spectacle, which will be broadcast on Netflix.