It is after a judge in August concluded that Google, in violation of the law, has monopolized the market for online searches.
"The playing field is not equal due to Google's behavior", writes the Justice Department in its lawsuit, according to CNN.
"This gap must be closed and Google must be deprived of these advantages".
In its application to the court, the department argues that the sale of Chrome, which is used on billions of devices worldwide, can help prevent an illegal monopoly from recurring.
Furthermore, the department wants the court to prohibit the exclusive, long-term agreements that Google has signed with, among others, Apple and Samsung, making Google the standard search engine on their devices.
Earlier in the week, media reported on the request based on information from sources, but now it is official. If Judge Amit Mehta grants the Justice Department's request, it could be the most far-reaching measure in the tech sector since American authorities attempted to break up Microsoft's dominance after the millennium shift.
The Justice Department's submission to the court means that a process will begin to gather facts, something that is expected to take several months. A hearing is scheduled for April next year, with a final decision to be expected later in 2025.