The development is alarming, according to the report. 162 journalists were killed during the period, an increase of 38 percent.
"In 2022 and 2023, a journalist was killed every fourth day, simply for doing their job", says UNESCO Director-General Audrey Azoulay in a statement.
UNESCO urges countries to do more to ensure that "crimes never go unpunished".
Most of the cases come from Latin America and the Caribbean: 61, while North America and Western Europe have had six cases of killed journalists during the two-year period. The majority of journalists were killed in 2023 in conflict zones, for the first time since 2017.
Almost all cases where journalists are killed on duty remain unsolved. Of the cases that UNESCO noted in 2006, 85 percent still lack a solution.