Mohammadi, who is currently temporarily released for medical care, took the opportunity to hold a speech to the French parliament via video link.
Her statement targeted sharp criticism against the political and religious leadership in Tehran.
Any negotiation with the Islamic Republic that does not include the fundamental rights of the Iranian population will only lead to the strengthening of religious tyranny, said the 52-year-old Mohammadi.
The human rights activist, who received the Nobel Peace Prize in 2023, has mostly been imprisoned over the past decade but received a time-limited medical parole in December.
She chose to appear without a veil, and said that she was well aware of the risks of criticizing the regime in Iran, but that it made "no difference being on one or the other side of the prison wall".
I believe in the necessity of ending the Islamic Republic (in Iran).