The protest, where thousands of demonstrators tried to block a motorway to the capital Tbilisi, was directed against the ruling party Georgian Dream and Prime Minister Irakli Kobachidze.
The two arrested politicians – Nika Melia, leader of the EU-friendly party Akhali, and Gigi Ugulava, mayor of Tbilisi – were two of the over 400 arrested after the police used both tear gas and water cannons to disperse the demonstrators.
The Ministry of the Interior had issued a warning before the demonstration that blocking motorways is punishable and can result in up to four years' imprisonment.
Both Melia and Ugulava have previously been imprisoned, which the opposition claims was politically motivated.
Georgian Dream has been accused in recent years of increasingly acting as a puppet to Russia. The party has, among other things, frozen negotiations on EU membership and recently talked about banning the political opposition altogether.
Last Monday, the EU revoked Georgian diplomats' right to enter the union without a visa, citing the country's repressive laws and the authorities' use of violence against peaceful demonstrations, politicians, and independent media.
