Mitoma, who injured one of his thigh muscles in connection with the Premier League match between Brighton and Wolverhampton, is not included in the World Cup squad that has now been presented.
Mitoma scored Japan's goal in the victory against England in March.
Another player missing is Takumi Minamino, who plays for Monaco. He has been out of action since December last year with a knee injury. Japan opens the World Cup against the Netherlands on June 14 and faces Sweden on June 26 in Dallas, Texas.
Facts: Japan's World Cup squad
Goalkeepers: Zion Suzuki, Parma, Keisuke Osako, Sanfrecce Hiroshima, Tomoki Hayakawa, Kashima Antlers.
Defenders: Yuto Nagatomo, FC Tokyo, Shogo Taniguchi, Sint-Truiden, Ko Itakura, Ajax, Tsuyoshi Watanabe, Feyenoord, Takehiro Tomiyasu, Ajax, Hiroki Ito, Bayern Munich, Ayumu Seko, Le Havre, Yukinari Sugawara, Werder Bremen, Junnosuke Suzuki, FC Copenhagen.
Midfielder/striker: Wataru Endo, Liverpool, Junya Ito, Genk, Daichi Kamada, Crystal Palace, Koki Ogawa, NEC Nijmegen, Daizen Maeda, Celtic, Ritsu Doan, Eintracht Frankfurt, Ayase Ueda, Feyenoord, Ao Tanaka, Leeds, Keito Nakamura, Reims, Kaishu Sano, Mainz, Takefusa Kubo, Real Sociedad, Yuito Suzuki, Freiburg, Kento Shiogai, Wolfsburg, Keisuke Goto, Sint-Truiden.
