The announcement from the European Tour, now the DP World Tour, comes less than a week after Spanish star Jon Rahm said he would not play on the LIV Tour in the future.
The players have to make a choice, says European Tour CEO Guy Kinnings.
Rahm and Englishman Tyrrell Hatton were part of Europe's most recent Ryder Cup team, despite being members of the LIV Tour.
According to the BBC, around 15 current LIV players, including Rahm and Hatton, have some form of membership status on the European Tour.
The future of LIV Golf is uncertain after the breakaway tour filed for bankruptcy protection in September, following the withdrawal of sponsorship by its Saudi financiers. Since then, financial firm BC Partners has announced that it is investing around three billion kronor (about $286 million) in the tour.
