The announcement was expected, but no less tough for Poland.
The striker and captain Robert Lewandowski will miss Sunday's European Championship opener against the Netherlands in Hamburg.
The notorious goal-scorer suffered an injury to his thigh in Monday's friendly against Turkey.
Even then, there were great concerns that the Barcelona star would not have time to recover from the problem in time for the opening match against the Netherlands.
Now it is clear that Lewandowski needs to rest for a few more days.
We hope he can return for the match against Austria, says Poland's national team coach Michal Probierz according to the AP news agency.
Poland is already missing Arkadiusz Milik, who normally plays for Juventus. The striker was injured against Ukraine in the penultimate friendly before the European Championship and has already undergone surgery on his injured knee.
The Netherlands is also struggling with injury concerns. National team coach Ronald Koeman lost an entire midfield before the European Championship: Frenkie de Jong, Teun Koopmeiners, and Marten de Roon, and had to call up Ian Maatsen and Joshua Zirkzee to cut their holidays short.
In the group's second match, France will face Austria on Monday.